Crime scene Lab
![Picture](/uploads/2/3/4/2/23426136/1382132621.png)
Summary:
In this crime a girl named Caroline was found dead in her boyfriends car. The police arrested brian but Brian said he was innocent. The police later noticed a blood stain on the car seat. They later found out that their was a car following them earlier which was her long time friend named Lauren. The only way that they could find the correct person that killed her is by doing a blood DNA test.
Who did it?
According to the DNA strands, the person who killed the cheerleader was Lauren which was suspect two , because the crime scene and the last suspect, which was Lauren matched.
3) No, their was no more solution remaining in tubes 1,2, 3 and 4 after loading the gel. The account for the solution actually remaining in these tubes is the data that could of been gathered would of not been gathered. What i mean by this is that their might of not been enough solution out into the gel and the solution would not spread or disperse in the run time.
4) The electric charge of the dyes that were put into the gel were negative. They are negative because during the process of the run time the dye would move toward the positive side of the box. The result at the end of the day is that positive and negative attract.
5. I think that the murderer killed the cheerleader because she was jealous of her. She might of been jealous of her being a cheerleader and of her having a boyfriend. The reason why i think she's jealous of her having a boyfriend was because she put the body in her boyfriends car.
Protocol:
1)Picked up solutions CS, V, S1 and S2 from the teacher
2)labeled 4 tubes 1,2,3 and 4 and added 3 uL DI water to each tube
3) Mixed solution CS by shaking the tube a couple of times and then transferred it to to tube one with a micro pipet
4) Mixed the solution V, then transferred it into the tube called two with a micro pipet
5) Mixed solution S1 , then pipetted 12 uL in the tube 3
6)Mixed solution S2 and then pipetted it into the tube numbered 4
7) After Lowered the gel tray and placed it into the electropherisis chamber. Made sure the wells of the gel were located toard the negative (black) electrode. This ensured that the samples move toward the positive (red) electrode when they are charged
8)Filled the chamber with 1X SB buffer solution to al level taht just covers the entire surface of the gel to a depth of 1-2 mm. The gel should be completely submerged under the buffer and wells should be filled with the buffer as well
9)We spinned thet tubes 1, 2, 3, and 4 in the microcentifrudge to pull all the solution to the bottom of the tubes
10) Set the micropipette to 15 uL and loaded each sample into separate wells. Used a fresh tip for each sample to avoid contamination. When we loaded each sample, we centered the pipette tip over the well and slowly expelled the sample. Used the other hand to support the pipette to void shaking.
11)We recorded each which sample we put in each well
12)Tightly closed the cover over the electrophoresis chamber and connected the electrical leads to the power supply
13) Turned the power voltage to 140-145 V
14)After 20 to 30 minutes the dye colors were identified, the power was turned off and it was unplugged, the gel was taken out by carefully removing the cover form the chamber while observing the dyes
In this crime a girl named Caroline was found dead in her boyfriends car. The police arrested brian but Brian said he was innocent. The police later noticed a blood stain on the car seat. They later found out that their was a car following them earlier which was her long time friend named Lauren. The only way that they could find the correct person that killed her is by doing a blood DNA test.
Who did it?
According to the DNA strands, the person who killed the cheerleader was Lauren which was suspect two , because the crime scene and the last suspect, which was Lauren matched.
3) No, their was no more solution remaining in tubes 1,2, 3 and 4 after loading the gel. The account for the solution actually remaining in these tubes is the data that could of been gathered would of not been gathered. What i mean by this is that their might of not been enough solution out into the gel and the solution would not spread or disperse in the run time.
4) The electric charge of the dyes that were put into the gel were negative. They are negative because during the process of the run time the dye would move toward the positive side of the box. The result at the end of the day is that positive and negative attract.
5. I think that the murderer killed the cheerleader because she was jealous of her. She might of been jealous of her being a cheerleader and of her having a boyfriend. The reason why i think she's jealous of her having a boyfriend was because she put the body in her boyfriends car.
Protocol:
1)Picked up solutions CS, V, S1 and S2 from the teacher
2)labeled 4 tubes 1,2,3 and 4 and added 3 uL DI water to each tube
3) Mixed solution CS by shaking the tube a couple of times and then transferred it to to tube one with a micro pipet
4) Mixed the solution V, then transferred it into the tube called two with a micro pipet
5) Mixed solution S1 , then pipetted 12 uL in the tube 3
6)Mixed solution S2 and then pipetted it into the tube numbered 4
7) After Lowered the gel tray and placed it into the electropherisis chamber. Made sure the wells of the gel were located toard the negative (black) electrode. This ensured that the samples move toward the positive (red) electrode when they are charged
8)Filled the chamber with 1X SB buffer solution to al level taht just covers the entire surface of the gel to a depth of 1-2 mm. The gel should be completely submerged under the buffer and wells should be filled with the buffer as well
9)We spinned thet tubes 1, 2, 3, and 4 in the microcentifrudge to pull all the solution to the bottom of the tubes
10) Set the micropipette to 15 uL and loaded each sample into separate wells. Used a fresh tip for each sample to avoid contamination. When we loaded each sample, we centered the pipette tip over the well and slowly expelled the sample. Used the other hand to support the pipette to void shaking.
11)We recorded each which sample we put in each well
12)Tightly closed the cover over the electrophoresis chamber and connected the electrical leads to the power supply
13) Turned the power voltage to 140-145 V
14)After 20 to 30 minutes the dye colors were identified, the power was turned off and it was unplugged, the gel was taken out by carefully removing the cover form the chamber while observing the dyes
Xylene Cyanol, Blue: .4 cm Distance traveled
Bromophenol Blue, Purple: .7 cm Distance traveled
Orange G, Orange: 1cm traveled
Bromophenol Blue, Purple: .7 cm Distance traveled
Orange G, Orange: 1cm traveled